iowa state university free software snapgene

#Iowa state university free software snapgene install

#Iowa state university free software snapgene install

A second machine is setup for running fragment analysis with either Liz or Rox standards.īYU users can send a request to the College of Life Sciences Computer Support group and ask that they install Geneious on your computer. For the faster configuration read lengths are shorter with an average QV20 of 650 bases. This machine can be configured to produce twice as many sample reads a day. In its current configuration, the ABI 3730xl will generate 1,152 (12 x 96) sample reads a day, with each read containing a QV20 read length of approximately 900 bases. Read lengths very from short to over 100Kb. We can run either instrument in CCS or CLR mode. An 8M SMRTcell can generate as much as 170Gbases of CLR DNA sequence data. Each 1M SMRTcell generates 15Gb of CLR DNA sequence data. Because the SMRT approach involves sequencing large, intact DNA segments, it dramatically improves or enables several genomics approaches, including: (1) de novo genome assembly, (2) detecting structural variants, (3) constructing full haplotypes, (4) more accurate transcriptome assembly, (5) detecting epigenetic modifications, and (6) direct RNA sequencing to measure post-transcriptional modifications and secondary RNA structure that is lost during cDNA synthesis. We are also running 2 Sequel instruments. In high output mode it generates some 2 billion reads per flow cell.

This machine can operate with twice this capacity in either Rapid Mode (2x2 lanes) or high output mode (2x8 lane).

In Rapid Mode generates 125-150Gb of sequence data (2x250) per lane of a 2 lane flow cell, or runs a single flow cell (8 lane flow cell) in “High Output Run Mode” and generates 62.5 Gb of sequence data (2x125) per lane.